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raji dc sign cells  (ATCC)


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    ATCC raji dc sign cells
    Raji Dc Sign Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3564 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raji+dc+sign+cells/Raji/10__3390_slash_polysaccharides6010018-77-16-39
    Average 99 stars, based on 3564 article reviews
    raji dc sign cells - by Bioz Stars, 2026-08
    99/100 stars

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    DC-SIGN positive cells in inflammatory infiltrate (arrows) of cutaneous leishmaniasis lesions caused by Leishmania ( Viannia ) braziliensis . Slide were stained with anti-human CD209 (A and C) followed by Dako Envision System. The slides were then developed with 3,3-diaminobenzidine (red-brown) and counterstained with Mayer’s hematoxylin. For control propose, anti-human CD209 antibody was omitted (B and D). Original magnification: 1000x (A and C), 400x (D) and 200x (B).

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania ( Viannia ) braziliensis and L. ( Leishmania ) amazonensis promastigotes

    doi: 10.1590/0074-02760220044

    Figure Lengend Snippet: DC-SIGN positive cells in inflammatory infiltrate (arrows) of cutaneous leishmaniasis lesions caused by Leishmania ( Viannia ) braziliensis . Slide were stained with anti-human CD209 (A and C) followed by Dako Envision System. The slides were then developed with 3,3-diaminobenzidine (red-brown) and counterstained with Mayer’s hematoxylin. For control propose, anti-human CD209 antibody was omitted (B and D). Original magnification: 1000x (A and C), 400x (D) and 200x (B).

    Article Snippet: RAJI cells and Leishmania culture - RAJI-transfected cells expressing DC-SIGN (DC-SIGN POS ) and RAJI-mock transfected (DC-SIGN NEG ) cells (Sigma Chemical Company, Saint Louis, USA) were maintained in culture in RPMI-1640 medium supplemented, with 2 mM L-glutamine (Gibco BRL, Gaithersburg, Germany), 100 U/mL penicillin (Sigma, USA), 100 µg/mL streptomycin (Sigma, USA) and 10% fetal bovine serum (FBS; Gibco BRL, Germany) (complete medium), at 37ºC in a humidified CO 2 incubator.

    Techniques: Staining

    representative flow cytometry histogram of DC-SIGN receptor analysis in RAJI cells in different time points of cell culture. Electronic gates R4 represents total RAJI transfected with DC-SIGN cells expressing DC-SIGN, R5 represents RAJI DC-SIGN POS cells expressing low amount of DC-SIGN (DC-SIGN Low ), and R6 represents RAJI DC-SIGN POS cells expressing high amount of DC-SIGN (DC-SIGN Hi ).

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania ( Viannia ) braziliensis and L. ( Leishmania ) amazonensis promastigotes

    doi: 10.1590/0074-02760220044

    Figure Lengend Snippet: representative flow cytometry histogram of DC-SIGN receptor analysis in RAJI cells in different time points of cell culture. Electronic gates R4 represents total RAJI transfected with DC-SIGN cells expressing DC-SIGN, R5 represents RAJI DC-SIGN POS cells expressing low amount of DC-SIGN (DC-SIGN Low ), and R6 represents RAJI DC-SIGN POS cells expressing high amount of DC-SIGN (DC-SIGN Hi ).

    Article Snippet: RAJI cells and Leishmania culture - RAJI-transfected cells expressing DC-SIGN (DC-SIGN POS ) and RAJI-mock transfected (DC-SIGN NEG ) cells (Sigma Chemical Company, Saint Louis, USA) were maintained in culture in RPMI-1640 medium supplemented, with 2 mM L-glutamine (Gibco BRL, Gaithersburg, Germany), 100 U/mL penicillin (Sigma, USA), 100 µg/mL streptomycin (Sigma, USA) and 10% fetal bovine serum (FBS; Gibco BRL, Germany) (complete medium), at 37ºC in a humidified CO 2 incubator.

    Techniques: Flow Cytometry, Cell Culture, Transfection, Expressing

    DC-SIGN expression in RAJI cells transfected or not with DC-SIGN during the 48 h in culture

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania ( Viannia ) braziliensis and L. ( Leishmania ) amazonensis promastigotes

    doi: 10.1590/0074-02760220044

    Figure Lengend Snippet: DC-SIGN expression in RAJI cells transfected or not with DC-SIGN during the 48 h in culture

    Article Snippet: RAJI cells and Leishmania culture - RAJI-transfected cells expressing DC-SIGN (DC-SIGN POS ) and RAJI-mock transfected (DC-SIGN NEG ) cells (Sigma Chemical Company, Saint Louis, USA) were maintained in culture in RPMI-1640 medium supplemented, with 2 mM L-glutamine (Gibco BRL, Gaithersburg, Germany), 100 U/mL penicillin (Sigma, USA), 100 µg/mL streptomycin (Sigma, USA) and 10% fetal bovine serum (FBS; Gibco BRL, Germany) (complete medium), at 37ºC in a humidified CO 2 incubator.

    Techniques: Expressing, Transfection

    binding analysis of Leishmania ( L. ) amazonensis (La) or L. ( Viannia ) braziliensis (Lb) promastigotes to RAJI DC-SIGN NEG or RAJI DC-SIGN POS cells after 2-48 h of co-culture. (A) Dotplot to double-staining analysis of Leishmania -CFSE (x axis) and DC-SIGN (CD209)-PE-Cy7 (y axis) from DC-SIGN NEG (left panels) or DC-SIGN POS (right panels) cells after 24 h of co-culture. The percentage of Leishmania -RAJI cells binding in different time points were analised for L. ( L. ) amazonensis (B) or L. ( V. ) braziliensis (C). * p < 0.05; ***p < 0.001.

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania ( Viannia ) braziliensis and L. ( Leishmania ) amazonensis promastigotes

    doi: 10.1590/0074-02760220044

    Figure Lengend Snippet: binding analysis of Leishmania ( L. ) amazonensis (La) or L. ( Viannia ) braziliensis (Lb) promastigotes to RAJI DC-SIGN NEG or RAJI DC-SIGN POS cells after 2-48 h of co-culture. (A) Dotplot to double-staining analysis of Leishmania -CFSE (x axis) and DC-SIGN (CD209)-PE-Cy7 (y axis) from DC-SIGN NEG (left panels) or DC-SIGN POS (right panels) cells after 24 h of co-culture. The percentage of Leishmania -RAJI cells binding in different time points were analised for L. ( L. ) amazonensis (B) or L. ( V. ) braziliensis (C). * p < 0.05; ***p < 0.001.

    Article Snippet: RAJI cells and Leishmania culture - RAJI-transfected cells expressing DC-SIGN (DC-SIGN POS ) and RAJI-mock transfected (DC-SIGN NEG ) cells (Sigma Chemical Company, Saint Louis, USA) were maintained in culture in RPMI-1640 medium supplemented, with 2 mM L-glutamine (Gibco BRL, Gaithersburg, Germany), 100 U/mL penicillin (Sigma, USA), 100 µg/mL streptomycin (Sigma, USA) and 10% fetal bovine serum (FBS; Gibco BRL, Germany) (complete medium), at 37ºC in a humidified CO 2 incubator.

    Techniques: Binding Assay, Co-Culture Assay, Double Staining

    binding analysis of Leishmania ( Leishmania ) amazonensis (La) or L. ( Viannia ) braziliensis (Lb) in RAJI DC-SIGN Low or RAJI DC-SIGN Hi populations after 2-48 h of co-culture. (A) Representatives histograms analysis of Leishmania -CFSE in DC-SIGN Low (left panels) or DC-SIGN Hi (right panels) after 24 h of co-culture. The percentage of Leishmania -CFSE binding to DC-SIGN Low or DC-SIGN Hi gated populations in different time points were analyzed for L. ( L. ) amazonensis (B) or L. ( V. ) braziliensis (C). * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania ( Viannia ) braziliensis and L. ( Leishmania ) amazonensis promastigotes

    doi: 10.1590/0074-02760220044

    Figure Lengend Snippet: binding analysis of Leishmania ( Leishmania ) amazonensis (La) or L. ( Viannia ) braziliensis (Lb) in RAJI DC-SIGN Low or RAJI DC-SIGN Hi populations after 2-48 h of co-culture. (A) Representatives histograms analysis of Leishmania -CFSE in DC-SIGN Low (left panels) or DC-SIGN Hi (right panels) after 24 h of co-culture. The percentage of Leishmania -CFSE binding to DC-SIGN Low or DC-SIGN Hi gated populations in different time points were analyzed for L. ( L. ) amazonensis (B) or L. ( V. ) braziliensis (C). * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: RAJI cells and Leishmania culture - RAJI-transfected cells expressing DC-SIGN (DC-SIGN POS ) and RAJI-mock transfected (DC-SIGN NEG ) cells (Sigma Chemical Company, Saint Louis, USA) were maintained in culture in RPMI-1640 medium supplemented, with 2 mM L-glutamine (Gibco BRL, Gaithersburg, Germany), 100 U/mL penicillin (Sigma, USA), 100 µg/mL streptomycin (Sigma, USA) and 10% fetal bovine serum (FBS; Gibco BRL, Germany) (complete medium), at 37ºC in a humidified CO 2 incubator.

    Techniques: Binding Assay, Co-Culture Assay